ORTHOGONAL POLYBIOSENSING AND IMAGING SYSTEMS

Toward forming a single hybrid biosensing-imaging system that can operate inside an incubator, structures and methods are directed to placing modular and removable biosensors and biocompatible interfaces in 3D transparent test wells that contain biological samples. The technology enables continuous monitoring of multiple simultaneous parameters and functions of a living cell or cell clusters suchas alterations of cellular ligands, physicochemical biomarkers, phenotypes, and/or extracellular compositions upon interactions with analytes or during progressions. Methods of capturing and analyzingdirect orthogonal information from biological samples in 2D and 3D, which are conducive to generating new insights are presented. 为了形成可以在培养器内操作的单一混合生物感测成像系统，结构和方法旨在将模块化和可移动的生物传感器以及生物相容性界面放置在3D透明测试井中，该3D透明测试井包含生物样本。该技术支持在与分析物交互时或在进展期间，活细胞或细胞簇的多个同时的参数和功能的连续监测，诸如细胞配体、理化生物标志物、表型和/或细胞外成分的变更。提出了从2D和3D生物样本中捕获和分析直接正交信息的方法，其有助于生成新的见解。