LAMP (loop-mediated isothermal amplification) primer group for rapidly detecting chicken derived components in beef and mutton, detection kit, detection method and application

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group for rapidly detecting chicken derived components in beef and mutton. The LAMP primer group comprises external primers Cb-F3 and Cb-B3 with sequences of SEQIDNO.1 and SEQIDNO.2 shown in the description, amplified cr...

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Bibliographische Detailangaben
Hauptverfasser: YU YUANYUAN, QU QINFENG, XU HONGBIN, GU WENJIA, ZHANG YINAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses an LAMP (loop-mediated isothermal amplification) primer group for rapidly detecting chicken derived components in beef and mutton. The LAMP primer group comprises external primers Cb-F3 and Cb-B3 with sequences of SEQIDNO.1 and SEQIDNO.2 shown in the description, amplified cross primers Cb-FIP and Cb-BIP with sequences of SEQIDNO.3 and SEQIDNO.4 shown in the description, and ring-forming primers Cb-FLP and Cb-BLP with sequences of SEQIDNO.5 and SEQIDNO.6 shown in the description. The invention further discloses an LAMP detection kit containing the LAMP primer group andapplication of the LAMP detection kit, and discloses a detection method for constant temperature amplification by using the LAMP primer group. The LAMP primer group disclosed by the invention is capable of quickly and accurately detecting whether deep-processed beef and mutton products contain chicken derived components or not, and has a good application prospect. 本发明公开了一种快速检测牛羊肉中鸡肉源性成分的LAMP引物组,所述的LAMP引物组包括序列如SEQ ID NO.1和