Culture method of mesenchymal stem cells after adipose cell cryopreservation and resuscitation

The invention relates to the technical field of biology, in particular to a culture method of mesenchymal stem cells after adipose cell cryopreservation and resuscitation. The extracted adipose tissueis treated and then cryopreserved at the deep low temperature; the cryopreserved adipose tissue is recovered to culture the adipose mesenchymal stem cells according to the usage amount in the later period, the cell culture is completed, and after the identification is qualified, the cell is transplanted with the resuscitated adipose tissue; compared with the existing adipose tissue cryopreservation and mesenchymal stem cell culture method, the method has the advantages that the operation is simple, the problems of repeated liposuction and adipose tissue-derived stromal cell culture are solved, the tissue and cell filtration is not needed, the problem that the adipose tissue-derived stromal cells enter digestive mucus and cannot be separated is solved by using the DNaseI, the number of theobtained stem cells is la