Method for preparing triphosadenine by immobilized enzyme method

The invention relates to the field of a biological technology, in particular to a method for preparing triphosadenine by an immobilized enzyme method. The method particularly comprises the following steps: (1) preparing ATP production enzyme; (2) immobilizing the ATP production enzyme; and (3) separating products. The ATP can be synthesized by adopting three kinds of ATP production enzyme such asadenosine kinase (EC 2.7.1.20, Ark), adenylate kinase (EC 2.7.4.3, Adk) and polyphosphoric acid-adenylate phosphotransferase (EC 2.7.4.-, Pap) and only through two-step enzymatic reaction, the reaction process is simple, the reaction is easy to control and the product quality is more stable; the ATP is prepared by an immobilized enzyme catalysis method, the immobilized enzyme can be used continuously and repeatedly for many times, and the production cost is greatly reduced; meanwhile, introduction of a large amount of pigment and other types of nucleotide impurities caused by the use of yeastis avoided and purificatio