Identification method of dead slaughtered beef

According to the invention, double-label detection is carried out on hemoglobin residue and peroxidase activity, the concentration C1 of peroxidase and the concentration C2 of hemoglobin in the sampleto be detected are determined, and by using a formula R=C2/C1*100%, a R value is obtained, and the standard threshold value of the R value is determined by collecting a large amount of dead beef dataof different parts due to different causes, so that the absolute standard for determining the dead beef is completed. By adopting the detection method disclosed by the invention, the to-be-detected sample is only subjected to single test with no need for limitation of the detection sample portion to determine whether the to-be-detected sample is dead slaughtered beef or slaughtered beef. 本发明通过对血红蛋白残留和过氧化物酶活性进行双标检测，同时测定待测样本中过氧化物酶的浓度C以及血红蛋白的浓度C，通过公式R＝C/C*100％，获得R值，并通过收集大量的因不同病因、不同部位的死宰牛肉的数据，确立了R值的标准阈值，从而完成了对死宰牛肉的判定的绝对标准。采用本发明的检测方法，仅对待测样品进行单次实验，不受检测样本部位的限制，就可以确定待测样本是死宰牛肉还是屠宰牛肉。