Method for determining enzyme activity

The invention is applicable to the technical field of enzyme detection and provides a method for determining enzyme activity. The method comprises the following steps: S1, performing recombinant protein purification on an enzyme digestion substrate of metalloproteinase; S2, performing an enzyme digestion reaction on the enzyme digestion substrate; and S3, reading a metal target plate after the enzyme digestion reaction, and calculating the enzyme digestion activity in a sample. Therefore, the method has the advantages of good sensitivity, good accuracy, simple operation, low price and wide application range. 本发明适用于酶检测技术领域，提供了一种测定酶活性的方法，包括以下步骤：S1：对金属蛋白酶的酶切底物进行重组蛋白纯化；S2：对酶彻底物进行酶切反应；S3：读取酶切反应后的金属靶板，并对样本内的酶切活性进行计算。借此，本发明灵敏度、准确性好，操作简便，价格便宜，应用范围广泛。