ONLINE DILUTION FOR A LIQUID CHROMATOGRAPHY SYSTEM USING A SAMPLE METERING PUMP

An apparatus for diluting a sample in a liquid chromatography system includes a metering pump, a sample needle and a sample valve. One of the sample valve ports is in fluid communication with the first pump port, a second one of the sample valve ports is in fluid communication with the second pump port, and a third one of the sample valve ports is in fluid communication with the sample needle. Thesample valve can be configured in a first to conduct a solvent to the first pump port and to fluidically terminate the second pump port, and in a second state in which the sample needle is in fluid communication with the second pump port. A merge valve configurable in different states is in fluid communication with the sample valve. The states of the sample valve and merge valve can be controlledto perform online dilution. 一种用于在液相色谱系统中稀释样品的设备包括计量泵、样品针和样品阀。样品阀端口中的一个样品阀端口与第一泵端口流体连通，样品阀端口中的第二个样品阀端口与第二泵端口流体连通，并且样品阀端口中的第三个样品阀端口与样品针流体连通。样品阀可被构造成在第一状态下将溶剂传导至第一泵端口并以流体方式终止第二泵端口，并且样品阀在第二状态下，样品针与第二泵端口流体连通。可在不同状态下构造的合并阀与样品阀流体连通。