Method for simultaneously measuring ACC007, lamivudine and tenofovir in plasma

The invention discloses a method for simultaneously measuring ACC007, lamivudine and tenofovir in plasma. The method comprises the following steps of pre-treating a plasma sample; performing quantitative determination by adopting a high-efficiency liquid chromatography tandem mass spectrum; and simultaneously analyzing and measuring the concentration of three drugs in plasma by using an internal standard quorum. The method has the advantages of high sensitivity, strong specificity, high precision, high accuracy, high stability, high extraction recovery rate, no obvious matrix effect and dilution effect and the like, and is suitable for simultaneously analyzing the amount of ACC007, lamivudine and tenofovir in plasma. 本发明公开了一种同时测定血浆中ACC007、拉米夫定和替诺福韦的方法，先对血浆样品预处理，再采用高效液相色谱串联质谱进行定量测定，利用内标法定量，同时对血浆中三种药物的浓度进行分析测定。该方法灵敏度高、特异性强、精密度好、准确度高、稳定性好、提取回收率高、无明显基质效应和稀释效应等优点，适用于同时分析血浆中ACC007、拉米夫定和替诺福韦的量。