Method for preparing guanosine triphosphate by using immobilized enzyme method

The invention relates to the technical field of biology and in particular to a method for preparing guanosine triphosphate by using an immobilized enzyme method. The method specifically comprises thefollowing steps: (1) preparing a GTP producing enzyme; (2) immobilizing the GTP producing enzyme; and (3) separating a product. In the method, two GTP producing enzymes including PPK and GMPK are adopted, and GTP can be synthesized by two-step enzymatic reactions; compared with a traditional tedious process for producing GTP by using a beer yeast, the method has the advantages that the reaction process is simpler, a reaction is easier to control, and the quality of a product is more stable; GTP is prepared by adopting an immobilized enzyme catalysis method, and an immobilized enzyme can be continuously and repeatedly used for many times, so that the production cost is greatly reduced; meanwhile, impurities such as pigments and other types of nucleotides introduced by using the yeast are avoided, so that purificati