Hybridoma cell line secreting anti-pseudosciaena crocea immunoglobulin T monoclonal antibody

The invention discloses a hybridoma cell line IgT-6 secreting an anti-pseudosciaena crocea immunoglobulin T (IgT) monoclonal antibody. The hybridoma cell line IgT-6 has a deposit number of CCTCC NO: C2019215. The hybridoma cell line IgT-6 is obtained by immunizing BALB/C mice four times with purified recombinant pseudosciaena crocea IgT heavy chain extracellular region protein antigen; taking spleen cells from mice with the highest titer of an antibody against the pseudosciaena crocea IgT, fusing the spleen cells with SP2/0 bone marrow cells, and screening. The monoclonal antibody has characteristics of strong specificity, high titer, good stability, etc., can be used for diagnosis of diseases and evaluation of vaccine use effects for pseudosciaena crocea, and has important theoretical andpractical significance for research and prevention of the diseases of pseudosciaena crocea. 本发明公开了一种分泌抗大黄鱼IgT单克隆抗体的杂交瘤细胞株IgT-6，保藏号为：CCTCC NO:C2019215。本发明以纯化的重组大黄鱼IgT重链胞外区蛋白抗原四次免疫BALB/C小鼠后；将针对大黄鱼IgT抗体效价最高的小鼠取脾细胞，与SP2/0骨髓细胞进行融