Real-time fluorescent PCR (polymerase chain reaction) detection primer probe set, kit and method for African swine fever virus CD2V gene

The invention belongs to the technical field of molecular biology, and discloses a real-time fluorescent PCR (polymerase chain reaction) detection primer probe set, kit and method for African swine fever virus CD2V gene A nucleotide sequence of a forward primer of the primer probe set is shown as SEQ ID NO. 4, a nucleotide sequence of a reverse primer is shown as SEQ ID NO. 5, and a nucleotide sequence of a specific fluorescent probe is shown as SEQ ID NO. 6, wherein a fluorescent reporter group is marked at the 5' end of the specific fluorescent probe, while a fluorescent quenching group is marked at the 3' end of the specific fluorescent probe. The primer probe set and kit provided by the invention help quickly, conveniently, efficiently and specifically detect whether a sample containsAfrican swine fever virus and whether the sample is identified as CD2V gene deletion, and are suitable for clinical identification detection. 本发明属于分子生物学技术领域，公开了一种非洲猪瘟病毒CD2V基因的实时荧光PCR检测引物探针组、试剂盒及检测方法。本发明的引物探针组的正向引物核苷酸序列如SEQ ID