Preparation method and application of attenuated goose astrovirus

The invention provides a preparation method of attenuated goose astrovirus and a live vaccine prepared by applying the attenuated goose astrovirus, and belongs to the technical field of waterfowl disease prevention and control. A method combining high-temperature treatment and low-temperature incubation is adopted to attenuate the virus. The method has the advantages of short virus attenuation time, low cost and long immune protection time. There is virulence return after multiple passages, thus effectively preventing goose gout disease caused by goose astrovirus. 本发明提供了一种鹅星状病毒弱毒的制备方法以及应用鹅星状病毒弱毒制备的活疫苗，属于水禽疾病预防与防控技术领域，本方法采用高温处理和低温孵育相结合的方法致弱病毒，该方法致弱病毒时间短，成本低，免疫保护时间长，且经多次传代后，没有毒力返强，可有效的预防鹅星状病毒引起的鹅痛风病。