Artificially modified PCV2 (Porcine Circovirus 2) Rep' protein, ELISA assay kit and application thereof

The invention provides an artificially modified PCV2 (Porcine Circovirus 2) Rep' protein, an ELISA assay kit and application thereof. The amino acid sequence of the PCV2 Rep' protein is shown as SEQ ID NO.1; and an ELISA plate is coated by the PCV2 Rep' protein, and an iELISA (Indirect ELISA) assay kit capable of being used for detecting antibodies in bodies of PCV2 naturally infected pigs or PVC2inactivated virus vaccine immunized pigs is built. The kit is combined with a PVC2 Cap protein antibody iELISA kit for use, and the serum antibodies of the pigs naturally infected by the PVC2 (or theserum antibodies of the pigs immunized by PCV2 inactivated virus vaccines) and the serum antibodies of the pigs immunized by inoculating PVC2 Cap protein subunit vaccines can be distinguished. 本发明提供了一种人工改造的猪圆环病毒2型Rep′(PCV2 Rep′)蛋白、ELISA检测试剂盒及其应用。该PCV2 Rep′蛋白的氨基酸序列如SEQ ID NO.1所示；用该PCV2 Rep′蛋白包被ELISA酶标板，构建成能够检测PCV2自然感染猪或PCV2全病毒灭活疫苗免疫猪体内抗体的间接ELISA(iELISA)检测试剂盒。该试剂盒结合PCV2 Cap蛋白抗体iELISA试剂盒一起使用，就能区分自然感染PCV2病毒的猪的血清抗体(或免疫PCV2全病毒灭