Preparation method of brain acellular stent

The present invention provides a preparation method of a brain acellular stent. The preparation method is characterized by comprising the following steps: (1) adopting a detergent soaking rinsing method to obtain a brain acellular stent as a cell carrier; (2) prevascularizing the brain acellular stent in the step (1) to obtain a prevascularized brain acellular stent; and (3) transplanting PC12 cells into the prevascularized stent prepared in the step (2) and verifying formation of a vascular network in the prevascularized brain acellular stent and importance of seed cell transplantation in tissue engineering. The preparation method provides experimental basis for the brain acellular stent as the cell carrier for repairing central nervous system injury, and simultaneously, the preliminarilyconstructed brain acellular stent prevascularizing provides a new treatment strategy for transplanting the brain acellular stent-carrying seed cells after prevascularization to tissue and nerve regeneration and repair after