Method for removing cells in organism tissues under a non-denature condition

The invention discloses a method for removing cells in organism tissues under a non-denature condition. The method comprises the following steps: placing an obtained organism tissue material in buffersolution, adding endonuclease, carrying out reaction at 25.0-40.0 DEG C, slowly adding saline solution in the reaction process, and maintaining the pH value at 5-7. The method described in the invention can remove cells and histocompatibility antigens in allogeneic tissues or xenogeneic tissues, and the method can achieve the maximum retention of growth factors beneficial to the growth of the cells and the tissues in the tissues while removing the cells and histocompatibility antigens in the tissues. 本发明公开了一种在非变性情况下脱去生物体组织中细胞的方法，将获取的生物体组织材料置于缓冲液内，加入核酸内切酶，25.0℃～40.0℃进行反应，反应过程缓慢加入盐溶液，并维持pH值在5～7之间。本发明所述的方法可以去除同种异体组织或异种异体组织中细胞及组织相容性抗原，且该方法在去除组织中细胞及组织相容性抗原的同时，最大程度的保留组织中对细胞及组织生长有益的生长因子。