Chromosome karyotype analysis method for roundleaf Cinnamomum Kanehirae Hay based on young leaf

The invention discloses a chromosome karyotype analysis method for roundleaf Cinnamomum Kanehirae Hay based on a young leaf. The chromosome karyotype analysis method comprises the following steps: material taking: collecting the young leaf of the Cinnamomum Kanehirae Hay in the sunny morning; pretreatment: placing the young leaf into a saturated p-xylene solution for pretreatment; fixation and storage; chromosome specimen preparation: using an enzymatic wall degradation hypotonic method, and subjecting to fixation, front hypotonic treatment, enzymolysis, rear hypotonic treatment and smearing to obtain a relatively good chromosome sample; staining: carrying out Giemsa staining; and microscopic examination. The method is simple and easy to operate; the chromosome karyotype analysis method for the roundleaf Cinnamomum Kanehirae Hay applied to in-situ PCR and FISH operations provides a technical basis for a cytological study of the Cinnamomum Kanehirae Hay; the invention first reports thechromosome karyotype anal