Method and kit for simultaneously detecting four inflammatory markers

The invention provides a method and a kit for simultaneously detecting four inflammatory markers. The kit for simultaneously detecting four inflammatory markers comprises: a microsphere reagent and abiotinylation antibody; wherein the microsphere reagent comprises four types of encoded microspheres, the four types of encoded microspheres respectively correspond to specific antibodies coupled to the four inflammatory markers, and the four types of inflammatory markers are PCT, IL-6, CRP and SAA; and the biotinylation antibody comprises detection antibodies for the four inflammatory markers, and the detection antibodies are labeled with fluorescein. The kit provided by the invention can simultaneously detect the concentrations of the four indicators of PCT, IL-6, CRP and SAA in the serum, thereby realizing the quantitative detection of the indicators, having high efficiency and reducing the cost, furthermore, the detection concentration range is wide, the required sample volume is small, and the variable coeffi