Electrochemical sensor for detecting mutation of H1047R locus of PIK3CA gene and preparation and application of electrochemical sensor

The invention belongs to the technical field of DNA point mutation detection, and specifically discloses an electrochemical sensor for detecting mutation of a H1047R locus of a PIK3CA gene and preparation and application of the electrochemical sensor. The electrochemical sensor is constructed based...

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Bibliographische Detailangaben
Hauptverfasser:	DING SHIJIA, ZHANG DECAI, QUE HAIYING, LIU PING, LI XINGRONG, GUO BIN, WANG TONG, PENG QILING, GAN XIUFENG, ZHAO MIN, YAN YURONG, CHENG WEI, WU HAIPING, ZHENG QINGYUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of DNA point mutation detection, and specifically discloses an electrochemical sensor for detecting mutation of a H1047R locus of a PIK3CA gene and preparation and application of the electrochemical sensor. The electrochemical sensor is constructed based on an NsbI enzyme and a quadruplet DNA self-assembly structure. The electrochemical sensor can detect the mutation of a PIK3CAH1047R gene sensitively and characteristically, is high in stability, good in reproducibility and low in cost, is expected to be applied to the measurement of actual samplesand clinical specimens, and is developed into a sensor with clinical application value. 本发明属于DNA点突变检测技术领域，具体公开了一种检测PIK3CA基因H1047R位点突变的电化学传感器及其制备与应用，本发明基于NsbI酶和四联体DNA自组装结构构建了一种电化学传感器，所述电化学传感器可以灵敏、特异地检测PIK3CA基因突变，且稳定性强，重现性好，成本低，有望应用于实际样品和临床标本的测定，发展成为具有临床应用价值的传感器。