microRNA related with reproduction functions, of Liaoning bred white cattle, and obtaining method of microRNA

The invention belongs to the technical field of biology, and particularly relates to microRNA related with reproduction functions, of Liaoning bred white cattle, and an obtaining method of the microRNA. Through cloning IGF-1 and PRL genes of the Liaoning bred white cattle, and through analysis on bioinformation and screening on target microRNA, the microRNA related with reproduction functions, ofLiaoning bred white cattle is obtained, and particularly, bta-miR-365-3p and bta-miR-2393, as well as bta-miR-365-3p and bta-miR-2393 respectively participate in adjustment and control of IGF-1 and PRL gene expression, work in IGF-1 and PRL gene expression and are related to reproduction properties of the Liaoning bred white cattle. 本发明属于生物技术领域，更具体而言，涉及辽育白牛与繁殖功能相关的microRNA及其获得方法。通过对辽育白牛IGF-1与PRL基因的克隆，并对其进行生物信息分析及靶标microRNA的筛选，获得辽育白牛与繁殖功能相关的microRNA，具体为bta-miR-365-3p和bta-miR-2393，bta-miR-365-3p和bta-miR-2393分别参与IGF-1和PRL基因表达的调控，在IGF-1和PRL基因表达中起作用，且与辽育白牛繁殖性能有关。