Sample pretreatment method and detection method of aflatoxin B1 in Pu'er tea by liquid chromatography-mass spectrometry

The invention provides a sample pretreatment method and detection method of aflatoxin B1 in Pu'er tea by liquid chromatography-mass spectrometry and belongs to the technical field of analytical chemistry. The sample pretreatment method provided by the invention includes the following steps: aflatoxin B1 in a Pu'er tea sample is extracted by an extraction solvent to obtain an extract; the extraction solvent is a mixed solution of acetonitrile and water, the extract is mixed with graphitized carbon black, a multi-wall carbon nanotube and an N-propyl ethylenediamine solid phase adsorbent, and purification is performed to obtain a to-be-measured sample. The extraction solvent used by the invention can fully extract the aflatoxin B1 in the Pu'er tea sample, the graphitized carbon black, the multi-wall carbon nanotube and the N-propyl ethylenediamine solid phase adsorbent are used together during the purification process, dispersive solid-phase extraction is performed, and impurities can beremoved. In addition, the