Method for detecting stearic acid residues in human serum albumin

The invention discloses a method for detecting stearic acid residues in human serum albumin, and belongs to the field of component detection. The method for detecting the stearic acid residues in thehuman serum albumin is characterized by comprising the steps of S1, preparing a stearate reference so...

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Hauptverfasser: ZHANG BAOXIAN, WAN XIAOFENG, HE YINGXIA, LI YA, YANG LIU, ZHOU KANGSEN, GUO XINYI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for detecting stearic acid residues in human serum albumin, and belongs to the field of component detection. The method for detecting the stearic acid residues in thehuman serum albumin is characterized by comprising the steps of S1, preparing a stearate reference solution with known content, and weighing a certain amount; S2, preparing a test solution, namely, weighing a human serum albumin sample, reacting with an organic solution of which the solute is an alkaline substance and the solvent is an organic matter 1, reacting with an organic solution of which the solute is boron trichloride and the solvent is the organic matter 1, finally extracting by using an organic solvent 2, and weighing the same amount as the reference solution after the preparation,wherein the reaction product of the organic matter 1 and stearic acid is stearate in the reference solution; and S3, respectively injecting the reference solution and the test solution into a gas chromatograph to obtain a chro