Measuring method of fungal hypha biomass

The invention belongs to the technical field of hypha biomass measuring methods, and particularly relates to a measuring method of fungal hypha biomass. The measuring method comprises the following steps of (1) glass slide treatment: sticking a medium paper sheet soaked by a base solution on a potat...

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Hauptverfasser: CAI JINGYUN, YI ZHUGANG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention belongs to the technical field of hypha biomass measuring methods, and particularly relates to a measuring method of fungal hypha biomass. The measuring method comprises the following steps of (1) glass slide treatment: sticking a medium paper sheet soaked by a base solution on a potato substrate sheet, then sticking the potato substrate sheet on a circular glass slide, placing the circular glass slide in a culture dish, filling the base solution into the culture dish until the medium paper sheet is submerged, covering the culture dish with a cover, and sterilizing at high pressure; (2) inoculated element treatment: sterilizing the inoculated elements under high pressure, cooling to room temperature, and soaking with a pure fungus culture solution; (3) inoculating and culturing; and (4) detection: culturing for several days, separating the medium paper sheet with the hyphae from the potato substrate sheet by using a pair of tweezers, dehydrating, drying and weighing. Thehypha biomass measuring m