Rapid detection method of ochratoxin A based on PEI

The invention discloses a rapid detection method of ochratoxin A (OTA) based on PEI, and belongs to the field of ochratoxin A detection. The method comprises the following steps: adding OTA with different standard concentrations into an MES buffer solution containing 0.05% PEI, taking OTA concentrat...

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Hauptverfasser: LI TAIHUA, HUANG LEI, GONG LEI, HU RUNTAO
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a rapid detection method of ochratoxin A (OTA) based on PEI, and belongs to the field of ochratoxin A detection. The method comprises the following steps: adding OTA with different standard concentrations into an MES buffer solution containing 0.05% PEI, taking OTA concentration as an x axis, taking a relative fluorescence value as a y axis to serve as a standard curve, andcalculating a regression equation and correlation coefficients; adding a sample to be tested into the MES buffer solution containing PEI, and substituting the measured relative fluorescence value into the regression equation to obtain ochratoxin A concentration in the sample to be tested. The concentration of the MES buffer solution is 20 mmol/L, and the pH of the MES buffer solution is 6. According to the rapid detection method, based on an enhancement effect of the surfactant PEI on an OTA fluorescence spectrum, the OTA is rapidly detected, the operation steps are simple, and the material is cheap and easy to obtai