Multiple PCR method for improving amplification specificity and uniformity

The invention belongs to the technical field of nucleic acid detection, and particularly relates to a multiple PCR method for improving amplification specificity and uniformity. The method comprises the steps as follows: extracting genome DNA from a sample; performing fragmentation and purification on the extracted genome DNA; carrying out a PCR amplification reaction by multiple specific primer pairs with the purified genome DNA as a template, wherein in the PCR amplification reaction, differential temperatures from low to high are used for annealing. The fragmentation and purification treatment is performed on DNA, the template and primers can be bond quickly, and the use rate of the primers is increased. Firstly, in a lower annealing temperature mode, amplification primers are fully bond to template DNA, the annealing temperature is increased gradually, and the binding specificity of all primers is improved; and amplification specificity and uniformity of the multiple PCR are remarkably improved. 本发明属于核酸检测