Method for culturing bone marrow mesenchymal stem cells of canines

The invention relates to the technical field of bone marrow mesenchyml stem cell culture, in particular to a method for culturing bone marrow mesenchymal stem cells (BMSCs) of canines. According to the method, the bone marrow mesenchymal stem cells are obtained by the processes of directly culturing collected canine bone marrow blood and the like. The steps of culturing the bone marrow mesenchymalstem cells are simplified; on the one hand, bone marrow blood cells do not need to be subjected to pretreatment including filtration, centrifugation and fat cell removal, and the method has the advantages of better maintaining original microenvironment of canine bone marrows, avoiding external interference, enabling contained matrix cells, extracellular matrix components and secreted factors to provide a comprehensive nutrient environment for mesenchymal stem cell growth; on the other hand, the method only needs a simple culture medium to select the adherent BMSCs, and does not need to separate hematopoietic stem cel