Detection method of linezolid product

The invention discloses a detection method of a linezolid product. According to the method, gradient elution is performed by adopting a mobile phase A and a mobile phase B for detection based on an HPLC. The mobile phase A is a solution formed by mixing a phosphoric acid aqueous solution with the vo...

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Hauptverfasser: XU LILI, WANG JINGYI, WANG LINLIN, SUN LIANFU, HU BAIYAN, WANG LICHUN, GUO JIN, HE CHUNRONG, ZHAO QINGYA, ZHANG LISHA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a detection method of a linezolid product. According to the method, gradient elution is performed by adopting a mobile phase A and a mobile phase B for detection based on an HPLC. The mobile phase A is a solution formed by mixing a phosphoric acid aqueous solution with the volume concentration of 0.08 to 0.12% and methanol according to a volume ratio of (80-90) to (20-10) or a 0.2 to 1g/L ammonium dihydrogen phosphate aqueous solution; and the mobile phase B is methanol. The method is simple in mobile phase configuration and is capable of analyzing more than twenty related substances with high separation degree. 本发明公开了一种利奈唑胺产品的检测方法。所述方法利用HPLC采用流动相A和流动相B进行梯度洗脱来进行检测,其中,所述流动相A为体积浓度0.08-0.12%的磷酸水溶液与甲醇以80-90:20-10的体积比混合的溶液,或者为0.2-1g/L磷酸二氢铵水溶液;和所述流动相B为甲醇。该方法的流动相配置简单,能够分析二十余种有关物质并且分离度高。