Method for detecting total flavonoids compound content in Ampelopsis grossedentata
The invention provides a method for detecting total flavonoids compound content in Ampelopsis grossedentata, and belongs to the technical field of the analysis detection. The method comprises the following steps: extracting Ampelopsis grossedentata sample by adopting an ethanol aqueous solution to o...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a method for detecting total flavonoids compound content in Ampelopsis grossedentata, and belongs to the technical field of the analysis detection. The method comprises the following steps: extracting Ampelopsis grossedentata sample by adopting an ethanol aqueous solution to obtain a to-be-detected sample solution; preparing a standard substance solution by taking (2R,3R)-dihydromyricetin as a standard substance; scanning the to-be-detected sample solution and the standard substance solution through full-wavelength by utilizing an ultraviolet visible spectrophotometer, and selectin the dual-wavelength detection by comparing an ultraviolet absorption spectrum of the to-be-detected sample solution with the ultraviolet absorption spectrum of the standard substance solution; and obtaining the content of the total flavonoids compound in the Ampelopsis grossedentata according to a standard curve and an absorbance value of the to-be-detected sample solution under the dual-wavelength detection |
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