Method for detecting total flavonoids compound content in Ampelopsis grossedentata

The invention provides a method for detecting total flavonoids compound content in Ampelopsis grossedentata, and belongs to the technical field of the analysis detection. The method comprises the following steps: extracting Ampelopsis grossedentata sample by adopting an ethanol aqueous solution to o...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: LIAO LUJING, AI LUNQIANG, LI YU, ZHANG YU, HE MEIJUN, HUANG DONGHAI
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention provides a method for detecting total flavonoids compound content in Ampelopsis grossedentata, and belongs to the technical field of the analysis detection. The method comprises the following steps: extracting Ampelopsis grossedentata sample by adopting an ethanol aqueous solution to obtain a to-be-detected sample solution; preparing a standard substance solution by taking (2R,3R)-dihydromyricetin as a standard substance; scanning the to-be-detected sample solution and the standard substance solution through full-wavelength by utilizing an ultraviolet visible spectrophotometer, and selectin the dual-wavelength detection by comparing an ultraviolet absorption spectrum of the to-be-detected sample solution with the ultraviolet absorption spectrum of the standard substance solution; and obtaining the content of the total flavonoids compound in the Ampelopsis grossedentata according to a standard curve and an absorbance value of the to-be-detected sample solution under the dual-wavelength detection