Method for preparing molybdenum disulfide/silver nano-immune substrate material, and repeatable immunodetection application thereof

The invention discloses a method for preparing a molybdenum disulfide/silver nano-immune substrate material, and a repeatable immunodetection application thereof. The method is characterized by comprising the following steps: 1) preparing a molybdenum disulfide nanomaterial; 2) spin coating a molybd...

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Bibliographische Detailangaben
Hauptverfasser:	GU CHENJIE, CHEN YING, JIANG TAO, ZHUANG WENTING, WANG FUYAN, JIANG YONG, NIU ZHIQIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	CHEMICAL SURFACE TREATMENT CHEMISTRY COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATIONOR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY IONIMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL COATING MATERIAL WITH METALLIC MATERIAL COATING METALLIC MATERIAL COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F DIFFUSION TREATMENT OF METALLIC MATERIAL INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION INGENERAL INORGANIC CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY PHYSICS SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THESURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION TESTING
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Beschreibung
Zusammenfassung:	The invention discloses a method for preparing a molybdenum disulfide/silver nano-immune substrate material, and a repeatable immunodetection application thereof. The method is characterized by comprising the following steps: 1) preparing a molybdenum disulfide nanomaterial; 2) spin coating a molybdenum disulfide nano-material solution on a clean silicon wafer, and after vacuum drying, sputteringa layer of Ag nanoparticles on the surface of the silicon wafer by using a magnetron sputtering method to obtain a molybdenum disulfide/silver nanomaterial; and 3) connecting an antibody to the molybdenum disulfide/silver nanomaterial to obtain the molybdenum disulfide/silver nano-immune substrate material. The immunodetection method of the molybdenum disulfide/silver nano-immune substrate material is as follows: dripping an antigen to be tested onto the molybdenum disulfide/silver nano-immune substrate material for incubation, dripping a gold nanorod immune probe solution and performing spectral measurement. After ca