Immune cell p16 detection kit

The invention discloses an immune cell p16 detection kit. The kit comprises a stationary liquid, a blocking endogenous peroxidase, a confining liquid, a washing liquid, a first antibody, a second antibody and a DAB developing solution, wherein the stationary liquid is paraformaldehyde with the mass concentration of 4%; the blocking endogenous peroxidase comprises methanol and 30% H2O2, and the volume ratio of methanol to 30% H2O2 is 100:1; the confining liquid comprises the following components in parts by weight: 55 parts of a PBS buffer solution, 5 parts of animal non-immune serum, 5 parts of cane sugar, 5 parts of tween-20, 1 part of Proclin300, 20 parts of skim milk powder and 100 parts of distilled water; the washing liquid comprises a PBS buffer solution with the pH value of 7.2; thefirst antibody is a goat anti-p16INK4a polyclonal antibody; and the second antibody is a horseradish peroxidase labeled goat anti-mouse IgG antibody. According to the invention, the staining effect of immune cell p16 detecti