Method for dynamically detecting unicellular exocrine protein secretion situation

The invention belongs to the technical field of exocrine protein detection, and particularly relates to a method for dynamically detecting a unicellular exocrine protein secretion situation. The method for dynamically detecting the unicellular exocrine protein secretion situation comprises the following steps that an antibody is immobilized, blocking is conducted, a biological sample is specifically captured, a nucleic acid labeled detection antibody is connected, fluorescent spots are shown, the fluorescent spots are micro dissected, nucleic acid fragments are digested, purification and amplification are conducted, and the nucleic acid fragments are detected and analyzed. According to the method for dynamically detecting the unicellular exocrine protein secretion situation, Elispot and nucleic acid sequence analysis are combined to form an ELISE method, the technical characteristics of being high in sensitivity, less in non-specific binding, high in throughput, and high in accuracy are realized, dozens or ev