Casein phosphopeptide and preparation method and application thereof

The invention relates to the field of polypeptides, in particular to casein phosphopeptide and its preparation method and application. In the invention, casein in cow's milk is used as a raw material.Casein phosphopeptide with strong calcium holding activity is prepared through trypsin and comp...

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Bibliographische Detailangaben
Hauptverfasser:	XIA ZUMENG, LUO HUJIE, LUO ZHEN, LI WENZHI, CHEN YUANYUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of polypeptides, in particular to casein phosphopeptide and its preparation method and application. In the invention, casein in cow's milk is used as a raw material.Casein phosphopeptide with strong calcium holding activity is prepared through trypsin and compound protease (trypsin and neutral protease) enzymolysis, enzyme inactivation, centrifugation, adjustment of isoelectric point/alcohol precipitation and impurity removal, and drying processes. The special cluster phosphoserine structure obtained by the invention has the ability of chelating calcium, andcan effectively promote the absorption and utilization of calcium. The molecular weight of the milk-derived casein phosphopeptide prepared by the invention is mainly between 1,000 and 5,000, which isbeneficial to human body absorption. The content of casein phosphopeptide is more than 85%, and its yield can reach 25%. The method is a preparation method with low cost and simple process, and is suitable for industrial produ