Establishment method of fingerprint spectrum of nacre-borneol-borax-boric acid eyedrops and fingerprint spectrum

The invention discloses an establishment method of a fingerprint spectrum of the nacre-borneol-borax-boric acid eyedrops and the fingerprint spectrum, and belongs to the technical field of traditionalChinese medicine analysis. High performance liquid chromatography is adopted in the method. Chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler ofa chromatographic column; the flow rate is 0.3-0.8mL/min; the column temperature is 20-40 DEG C; and the detection wavelength is 200-240nm. Gradient elution is adopted for a mobile phase, wherein 0.08-0.12% of TFA-water is used for the phase A, and 60-80% of acetonitrile aqueous solution and 0.08-0.1% of TFA water are adopted for the phase B. A fingerprint spectrum of the nacre-borneol-borax-boricacid eyedrops is obtained by gradient elution. The fingerprint spectrum provided by the invention can comprehensively represent the quality information of the nacre-borneol-borax-boric acid eyedrops,so that the quality of the