Method for rapidly detecting trans fatty acids in food

The invention discloses a method for rapidly detecting trans fatty acids in food, and the method comprises the following steps of taking 2-5g of samples for pulverizing and placing in a 100mL test tube, adding 10-20ml of water for uniform mixing, adding 10-20ml of mixed acid, placing the test tube in a water bath of 60-80 CEG D for heating for 30-60min, adding 10-20 ml of acetonitrile-ethanol mixture for uniform mixing and shaking for 3-5min, adding 10-15ml of n-ethane and petroleum ether for reflux and extraction, evaporating an organic solvent to obtain sample oil; placing 60ml of the sampleoil in PE test tube, adding 5-10ml of isooctane and 0.2-0.5ml of sodium hydroxide-methanol solution for shaking for 5-10min, mixing in test tube and standing for clarification, adding sodium bisulfate monohydrate for vortex for 2min and standing, taking the supernatant for testing; and sending the supernatant to the gas chromatography-mass spectrometer for analysis. The method provided by the invention can completely sep