K252A fermentation medium and preparation method thereof

The invention discloses a K252A fermentation medium and a preparation method thereof. According to the fermentation medium, by means of optimized ingredient matching, and addition of choline chloride,disodium succinate, methionine and other functional factors, not only can nutrition be provided for nocardiopsis growth and metabolism, but also an intermediate is provided for K252A biosynthesis after metabolic medium, and finally a high-yield K252A fermentation broth is obtained. By using the fermentation broth, flask shaking fermentation is conducted for 9 days, and the K252A titer is higher than 2.60 g/L. By controlling two key technological conditions of the pH and dissolved oxygen, and optimizing the feed supplement control strategy of glucose and functional factors at the same time, culture is conducted for about 264 hours, the K252A titer can reach 5.79 g/L, the level is significantly improved compared with that reported in the literature, and the basis of industrial production isachieved. 本发明公开了一种K252A发酵