Multiplex PCR detection primer set and kit used for mycoplasma gallisepticum, mycoplasma gallisepticum and parabacterium bacillus and application of kit

The invention provides a multiplex PCR detection primer set and kit used for mycoplasma gallisepticum, mycoplasma gallisepticum and parabacterium bacillus and application of the kit, and belongs to the technical field of molecular detection. The primer set comprises MS-F, MS-R, MG-F, MG-R, APG-F and...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ZHAO SHIHUA, CHEN WEI, LU YAN, LIU WEI, ZHANG YUEMEI, SA NA, WANG NA, SONG YUE, QIAN LINNA, ZHANG FAN, DALAIBAOLIGE, DAI LINGLI, YANG BIN, SONG AIJUN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides a multiplex PCR detection primer set and kit used for mycoplasma gallisepticum, mycoplasma gallisepticum and parabacterium bacillus and application of the kit, and belongs to the technical field of molecular detection. The primer set comprises MS-F, MS-R, MG-F, MG-R, APG-F and APG-R; the nucleotide sequences of MG-F, MG-R, MS-F, MS-R, APG-F and APG-R are shown in SEQ ID NO:1-SEQ ID NO:6 respectively. It is verified that the primer set has high specificity and sensitivity. 本发明提供了一种用于鸡毒支原体、鸡滑液囊支原体和副鸡禽杆菌多重PCR检测引物组，属于分子检测技术领域，所述引物组包括MS-F、MS-R、MG-F、MG-R、APG-F和APG-R；所述MG-F、MG-R、MS-F、MS-R、APG-F和APG-R的核苷酸序列分别如SEQ ID NO：1～SEQ ID NO：6所示。经过验证，本发明的引物组具有良好的特异性和灵敏度。