Method for quantitatively detecting peanut components in shredded hazelnut stuffing by utilizing double digital PCR

The invention provides a method for quantitatively detecting peanut components in shredded hazelnut stuffing by utilizing double digital PCR. The method adopts a dual channel detection method to simultaneously detect two fluorescence signals by utilizing a digital PCR system; probes for detecting a peanut species-specific gene sequence and a hazelnut species-specific gene sequence are respectivelymarked as VIC and FAM; and the DNA copy number percentage of peanuts in hazelnuts and peanuts can be obtained through calculation through the copy number concentration of the peanut species-specificgene sequence and the hazelnut species-specific gene sequence measured in the same PCR reaction system, and the mass percentage of peanut components in hazelnut and peanut components can be obtained through conversion. The method can accurately and rapidly detect the mass percentage content of the peanut components in shredded hazelnut stuffing, so that accurate and reliable technical data can beprovided for the authentici