Test method for Gnalphaq gene expression characteristics of melanocytes treated with different concentrations of alpha-MSH

The invention discloses a test method for Gnalphaq gene expression characteristics of melanocytes treated with different concentrations of alpha-MSH. The method includes the following steps: isolationand purification of melanocytes; primer design and synthesis; extraction and identification of total...

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Bibliographische Detailangaben
Hauptverfasser:	LIANG RUI, LI ZHEN, PANG QUANHAI, BAI RUI, WANG TIANYUAN, PANG ZHIHUA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a test method for Gnalphaq gene expression characteristics of melanocytes treated with different concentrations of alpha-MSH. The method includes the following steps: isolationand purification of melanocytes; primer design and synthesis; extraction and identification of total RNA after treating melanocytes with different concentrations of alpha-MSH; reverse transcription and amplification of target fragment; purification and recovery of PCR amplified products; determination of a GnalphaqcDNA sequence; transformation of recombinant plasmids; plasmid DNA extraction; Gnalphaq mRNA qRT-PCR detection of melanocytes treated with different concentrations of alpha-MSH; Gnalphaq protein level detected after melanocytes treated with different concentrations of alpha-MSH; anddata processing and statistical analysis. According to the present invention, sheep melanocytes are separated and purified, and treated with different concentrations of alpha-MSH, and the results ofthe Real time PCR test and