Japanese B encephalitis virus standard plasmid constructing primer and application thereof

The invention discloses a Japanese B encephalitis virus standard plasmid constructing primer. The primer comprises a forward primer and a reverse primer. Besides, the invention provides an applicationof the primer to construction of Japanese B encephalitis virus standard plasmids. The application comprises the steps of performing inoculation and culture, extracting Japanese B encephalitis virus nucleic acid, designing the Japanese B encephalitis virus standard plasmids, performing a reverse transcription PCR reaction and constructing the Japanese B encephalitis virus standard plasmids. Besides, the invention provides a quantitative detection method of the Japanese B encephalitis virus standard plasmids. The invention provides the primer for constructing the Japanese B encephalitis virus standard plasmids which are good in specificity and high in sensitivity, and the method for quickly and quantitatively detecting the Japanese B encephalitis virus standard plasmids is established. 本发明公开了一种乙脑病毒标准品质粒构建引物，该引物包括正向