Separation method and application of edible mushroom agarics basidiospores

The invention relates to a separation method and application of edible mushroom agarics basidiospores. The separation method comprises the steps of selecting parents, collecting basidiospores, obtaining pure mycelia, confirming monocaryon hypha and dicaryon hypha and the like. According to the separation method, the basidiospores are collected simply and quickly through a physical mode, so that the process of collecting the basidiospores is shortened from 2 days to 20-30min, the factors, which make the basidiospores difficult to release, such as unsuitable growth and development time and temperature can be overcome, the pollution rate is reduced, and the basidiospores are obtained efficiently and conveniently. 本发明涉及一种食用菌伞菌类担孢子的分离方法和应用，包括以亲本的选择、担孢子的收集、纯菌丝体的获得和单双核菌丝确认等步骤；本发明以物理方式简易快速收集担孢子，使得担孢子收集过程由2天缩短至20-30min，且能够克服因生长发育时间、温度不适宜造成孢子难以释放的因素，减少了污染率，高效便捷地获得担孢子。