Method for removing endotoxin in oligomeric sodium hyaluronate

The invention discloses a method for removing endotoxin in oligomeric sodium hyaluronate, and the method comprises the following steps in sequence: (1) performing raw material endotoxin limit detection; (2) dissolving the oligomeric sodium hyaluronate in water; (3) respectively adding a sodium hydroxide solid and a sodium chloride solid, and stirring; (4) adjusting the pH of the solution to neutrality; and (5) adding ethanol for precipitation, dewatering and drying to obtain oligomeric sodium hyaluronate powder. The method has the advantages of thorough treatment, simple steps and low cost, and the endotoxin content of the treated oligomeric sodium hyaluronate reaches the medical standard (less than 0.05 EU/mg). 本发明公开了一种去除寡聚透明质酸钠中内毒素的方法，包括依次进行的如下步骤：（1）原料内毒素限度检测；（2）将寡聚透明质酸钠溶于水中；（3）分别加入氢氧化钠固体和氯化钠固体，搅拌；（4）调节溶液pH至中性；（5）加入乙醇沉淀，脱水，干燥，得到寡聚透明质酸钠粉末。本发明提供的方法处理彻底、步骤简单、成本低廉，且处理后的内毒素含量达到医用级标准（小于0.05 EU/mg）。