Method for detecting hydrogen peroxide and relevant target object on the basis of nanoprobe

The invention belongs to the technical field of biological detection, and discloses a method for detecting a hydrogen peroxide and a relevant target object on the basis of a nanoprobe. The method adopts a water-soluble rare earth doped NaCeF4 nanomaterial as a fluorescence probe, rare earth ion lumi...

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Hauptverfasser: YU SHAOHUA, LEI XIALIAN, LIU, GONG ZHONGLIANG, TU DATAO, CHEN XUEYUAN, YI XIAODONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of biological detection, and discloses a method for detecting a hydrogen peroxide and a relevant target object on the basis of a nanoprobe. The method adopts a water-soluble rare earth doped NaCeF4 nanomaterial as a fluorescence probe, rare earth ion luminescence is quenched through the oxidation-reduction reaction of the hydrogen peroxide and a ceriumion, and the change of doped rare earth ion fluorescence intensity is used for realizing the detection of hydrogen peroxide concentration. The method can be used for detecting the hydrogen peroxide instandard solution or reactants in an enzymatic reaction for generating the hydrogen peroxide, and the detection of the hydrogen peroxide, biological enzymes or zymolyte (such as uric acid) in serumscan be realized. The method has the advantages of being convenient in operation, good in anti-interference performance, quick, sensitive, economic, practical and the like, a theoretical foundation andtechnical support are provi