Molecularly imprinted polymer coated carbon dot fluorescence probe, and preparation method and application thereof

The invention discloses a molecularly imprinted polymer coated carbon dot fluorescence probe, and a preparation method and an application thereof. A hydrothermal process is adopted to prepare c-dots,the surface of the c-dots is grafted with silica, and a molecularly imprinted polymer is in situ sealed in the pores of the silica (mip@c-dots). The fluorescence probe of the invention is successfullyapplied to AChE activity detection, has the characteristics of high sensitivity, rapidness in response, and simplicity in operation, and is of great significance in the fields of pathogenesis research, disease diagnosis and new drug development of the Alzheimer's disease. 本发明公开了一种分子印迹聚合物包覆碳点荧光探针及其制备方法和应用，本发明采用水热法制备c-dots，表面接枝二氧化硅，并用分子印迹聚合物原位密封在二氧化硅孔(mip@c-dots)中。本发明的荧光探针已成功应用于AChE活性检测，具有灵敏度高、响应快速、操作简便等特点，在阿尔茨海默病的发病机理研究、疾病诊断和新药开发等方面具有重要意义。