Hypochlorous acid fluorescence probe based on FRET mechanism

The invention discloses a hypochlorous acid fluorescence probe based on an FRET mechanism. The probe uses a coumarin fluorophore as an energy donor and thiomethylbenzene as an energy acceptor, and canrealize the ratio-dependent detection by the FRET mechanism. The probe can selectively react with HClO, and with the increase of the concentration of HClO, the fluorescence intensity gradually increases at 480 nm and gradually decreases at 550 nm. The probe can quickly respond to HClO, and has great application values in the field of biochemistry. 本发明公开了一种基于FRET机制的次氯酸的荧光探针,该探针以香豆素荧光团为能量供体，硫甲基苯为能量受体，通过FRET机制可实现比率型检测。该探针能选择性与HClO作用，随着HClO浓度的增加，其荧光强度在480 nm处逐渐增强，在550 nm处逐渐减弱。该探针能够快速与HClO响应,在生物化学领域具有重要的应用价值。