Method for determining content of fruit acid in akebia trifoliata

The invention discloses a method for determining the content of fruit acid in akebia trifoliate. The method includes the following steps of: accurately weighing 10.84mg of an akebia trifoliate fruit acid reference substance to a 10mL volumetric flask, dissolving the substance in methanol and diluting to the scale, and further preparing a reference substance stock solution with a concentration of 1.084mg.mL-1; accurately weighing 3g of product powder, placing the product powder in a 50ml conical flask, accurately adding 50ml of methanol, weighing, carrying out heating reflux for 40min, placingat room temperature, making up the weight with methanol, uniformly shaking, filtering with a microporous filtration membrane, and collecting subsequent filtrate to obtain the solution; separately injecting the reference substance solution and a test sample solution into a chromatographic column for detection, determining the peak area integral value, performing calculation by the peak area according to an external standar