L-pantoyl lactone dehydrogenase derived from Cnuibacter physcomitrellae

The invention relates to the technical field of genetic engineering, particularly relates to L-pantoyl lactone dehydrogenase and application thereof, and discloses L-pantoyl lactone dehydrogenase derived from Cnuibacter physcomitrellae for the first time. The L-pantoyl lactone dehydrogenase has excellent enzyme activity on L-pantoyl lactone and can be applied to catalytic synthesis of D-pantoyl lactone. A system for chiral inversion synthesis of D-pantoyl lactone with L-pantoyl lactone by multi-enzyme cascade catalysis is constructed, and a genetically engineered bacterial cell co-expressing L-pantoyl lactone dehydrogenase, D-ketopantolactone reductase and glucose dehydrogenase is used as a catalyst, accumulation and spontaneous hydrolysis of the intermediate product ketopantolactone is avoided, and the steps of separating the intermediate product, racemizing the L-pantoyl lactone, lactonizing D-pantoic acid under an acidic condition are omitted, so that the reacting process is simplified, use of acid and alk