L-panolactone dehydrogenase derived from Nocardia asteroides and application of L-panolactone dehydrogenase

The invention relates to the technical field of genetic engineering, in particular to L-panolactone dehydrogenase and application thereof. The L-panolactone dehydrogenase derived from Nocardia asteroides is disclosed for the first time, and the L-panolactone dehydrogenase has good enzymatic activity to L-panolactone, and can be applied to catalytic synthesis of D-panolactone. A system of multi-enzyme cascade catalysis for chiral overturning of the L-panolactone to synthesize the D-panolactone is established, genetically engineered bacterium cells for co-expression of the L-panolactone dehydrogenase, D-ketopantolactone reductase and glucose dehydrogenase are taken as a catalyst, accumulation and spontaneous hydrolysis of an intermediate product, namely ketopantolactone are avoided, the steps of separation of the intermediate product, racemization of the L-panolactone, lactonization of D-pantoic acid under the acid condition and the like are omitted, the reaction process is simplified, using of acid and base is