Preparation of anti-CD20 nanobody

The invention discloses a VHH chain of a nanobody of CD20, discloses FR amino acid sequences selected from a following group in a framework region (FR) and amino acid sequences of a complementary determining region (CDR), further discloses a CD20 nanobody, further discloses a DNA molecule, further discloses a host cell, and further discloses application of the CD20 nanobody to detection of the CD20. The VHH chain comprises the framework region (FR) and the complementary determining region (CDR), the DNA molecule encodes the VHH chain of the nanobody of the CD20 or the CD20 nanobody, and the host cell can express the nanobody of the CD20. Through the gene sequences of the nanobody and the host cell, the nanobody can be efficiently expressed in Escherichia coli, and is applied to the research and development of CD20 detection reagents and biopharmaceuticals. 本发明公开了一种CD20的纳米抗体的VHH链，包括框架区FR和互补决定区CDR，公开了框架区FR选自下组的FR的氨基酸序列和互补决定区CDR氨基酸序列，本发明还公开了一种CD20纳米抗体，还公开了一种DNA分子，它编码本发明所述的CD20的纳米抗体的VHH链或本发明所述的CD20纳米抗体，还公开了一种宿主细胞