Human papillomavirus (HPV) primer and molecular beacon combination based on isothermal amplification, kit and detecting method

The invention belongs to the technical field of biology, and discloses a primer and molecular beacon combination for quickly detecting human papillomaviruses (HPVs) based on an isothermal amplification technology, a kit and a detecting method. The primer and molecular beacon combination is shown as SEQ ID No.1-17, and can specifically recognize the 13 different types of high-risk HPVs and two types of medium-risk genotypes with the high incidence in Chinese population; and according to the kit and the method for real-timely detecting the HPVs through fluorescence based on the isothermal amplification technology, the 15 types of medium-and-high-risk HPVs can be detected within the short time (1.5 h), accuracy is good, sensitivity is high, repeatability is high, specificity is good, no nonspecific reaction with clinically similar pathogens exists, using is convenient, only an extracted template sample needs to be added into a reaction tube, the manual operation time is less than 5 min, the requirement of isothe