Cyslization derivative fluorescent probe as well as preparation method and application thereof

The invention relates to a cyslization derivative fluorescent probe as well as a preparation method and application thereof. The name of the compound is 2-cyano-3-(6-(dimethylamino)quinoline-2-yl) acrylate. The compound provided by the invention is small in molecular mass, simple in structure and capable of serving as a molecular sensor to sensitively and selectively detect sulfite in living cellsand start a fluorescence reaction. The detection mechanism is not affected by sulfide and a sulfhydryl compound and has the characteristic of high selectivity, what is more outstanding is that. The fluorescent molecular probe almost has no toxicity to cells , has a very good cell membrane permeation effect and is capable of permeating a living cell membrane within 2 min to start the fluorescencereaction. The cyslization derivative fluorescent probe provides a novel method for detecting endogenous sulfite of the living cell so as to have very important practical significance. 本发明涉及一种喹啉环类衍生物荧光探针及其制备方法和应用，化合物名称为2-氰基-3-(