Method for precisely detecting CEA by molecular imprinting combined with silent region internal standard SERS technology

The invention discloses a method for precisely detecting CEA by molecular imprinting combined with silent region internal standard SERS technology. The method comprises the steps of preparing gold-silver core-shell nanoparticles with an internal standard and polydopamine gold-plated nucleus core-shell nanoparticles with labeled signals, assembling the gold-silver core-shell nanoparticles on a goldplate as an SERS substrate, preparing a molecular imprinting layer containing CEA on the SERS substrate to obtain an SERS molecular imprinting layer capable of specifically recognizing CEA, specifically capturing the CEA in the solution by using CEA molecular imprinting, marking the captured CEA by using an SERS probe modified by an antibody, finally carrying out SERS detection, and estimating the concentration of CEA in the solution according to a ratio of silent region Raman signal intensity and the SERS probe and SERS substrate internal standard signal intensity. According to the method, the detection efficiency o